A binding motif for Siah ubiquitin ligase

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A binding motif for Siah ubiquitin ligase.

The Drosophila SINA (seven in absentia) protein and its mammalian orthologs (Siah, seven in absentia homolog) are RING domain proteins that function in E3 ubiquitin ligase complexes and facilitate ubiquitination and degradation of a wide range of cellular proteins, including beta-catenin. Despite these diverse targets, the means by which SINASiah recognize substrates or binding proteins has rem...

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Elucidation of the substrate binding site of Siah ubiquitin ligase.

The Siah family of RING proteins function as ubiquitin ligase components, contributing to the degradation of multiple targets involved in cell growth, differentiation, angiogenesis, oncogenesis, and inflammation. Previously, a binding motif (degron) was recognized in many of the Siah degradation targets, suggesting that Siah itself may facilitate substrate recognition. We report the crystal str...

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Structure and ubiquitin binding of the ubiquitin-interacting motif.

Ubiquitylation is used to target proteins into a large number of different biological processes including proteasomal degradation, endocytosis, virus budding, and vacuolar protein sorting (Vps). Ubiquitylated proteins are typically recognized using one of several different conserved ubiquitin binding modules. Here, we report the crystal structure and ubiquitin binding properties of one such mod...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 2003

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.0534783100